Product: Anti-Fas Ligand mAb, clone 2C101
Cat. No.: MC-137 (100 μg)
Synonyms:
CD95L, APO-1L, CD178, TNFSF 6
Background:
Fas (CD95/APO-1) Ligand (FasL) is a 43 kDa
type II membrane protein and member of the
tumor necrosis factor family. Human FasL can
be released from cells as a 26 kDa soluble
protein upon cleavage by metalloproteinases.
FasL is expressed on various cells including T
cells, NK cells, Monocytes, Sertoli cells and
tumor cells. Interaction of FasL with its receptor
triggers rapid apoptosis. FasL-induced
apoptosis is involved in T cell receptor/anti-CD3
induced cell death, cytotoxic T cell activity,
maintainence of immune privilege, immune
escape of tumor cells and viral hepatitis.
Malfunction of the Fas system causes
hyperplasia.
Specificity:
This antibody is specific for the extracellular
domain of human Fas Ligand.
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
Recombinant human FasL (extracellular domain)
Format:
100 μg of 1 mg/mL affinity-purified monoclonal
antibody. Prepared in 0.15M PBS, pH 7.2 with
protein stabilizer. Purity
95% by SDS-PAGE.
Storage:
Store at 4°C. Do not freeze.
Applications and Suggested Dilutions:

Functional Activity: Inhibits apoptosis in anti-
CD3 stimulated Jurkat T-cells ( 0.5 ~ 5 μg/mL)
suitable. Excellent for neutralization.

Flow cytometry: 1 x 106 H9 T-cells stained
with 1 μg/mL antibody, followed by
biotinylated goat anti-mouse IgG and
streptavidin-PE.

Immunocytochemistry
Suggested starting dilution is >1:100
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.