Cat. No: PC-153 (100 μg)
Description:
Chromatin condensation and nuclear
fragmentation (CCNF) is the hallmark of
apoptosis. CCNF is triggered by the activation of
members of the caspase family, caspase
activated Dnase (CAD/DFF40), and several
novel proteins including AIF and CIDE[1]. A new
inducer of chromatin condensation was recently
identified and designated acinus (for apoptotic
chromatin condensation inducer in the nucleus).
Acinus is cleaved by caspase-3 and an
additional unknown protease generating a small
active peptide p17, which causes chromatin
condensation in vitro when it is added to purified
nuclei. Acinus also induces apoptotic chromatin
condensation in cells. Acinus is ubiquitously
expressed. Three different spliced forms of
acinus have been identified in human and
mouse and designated acinusL, and acinusS,
and acinusS’[2].
Specificity:
Recognizes human acinus. Detects a band of
~220 kDa by Western blot.
Species Reactivity:
Human, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
Synthetic peptide corresponding to aa 1329-
1341 of human AcinusL, aa 602-614 of human
AcinusS’ and aa 571-583 of human AcinusS.
This sequence is identical to that of mouse
acinus.
Format:
100 μg of purified IgG in 200 μl PBS containing
0.02% sodium azide.
Storage and Stability:
Store at +4°C.
Applications and Suggested Dilutions:

Western blot: 0.25 – 0.5 μg/ml.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of Acinus in K562
whole cell lysate with anti-Acinus (CT) at 0.5
μg/ml.
References:
1. Kroemer, G. & Zamzami, N.; Condensed
matter in cell death; Nature 401, 127(1999).
2. Sahara, S., et al; Acinus is a caspase-3
activated protein required for apoptotic
chromatin condensation; J. Cell Biol. 143,
225 (1998).
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.