Product: Anti-Keratin 5 & 8 mAb, clone RCK102
Cat. No.: MC-961 (1 mL)
Description:
This antibody, directed against cytokeratins 5
and 8, reacts with virtually all epithelial cell types
and epithelial tumors. No reaction is observed
in non-epithelial tissues. Therefore this
monoclonal antibody can be used for the
discrimination of carcinomas and non-epithelial
tumors such as lymphomas, sarcomas and
neural tumors. This antibody is applicable on
frozen and paraffin-embedded tissue sections.
Embedding and formalin fixation may give
antigen denaturation or masking. Therefore
results on paraffin sections should be evaluated
cautiously. The antibody is also useful for
staining of cell lines and cytological specimens.
Specificity:
Recognizes keratin 5 and 8 (58 and 52.2 kDa
cytokeratin proteins, respectively).
Species Reactivity:
Human. Cross-reacts with most mammals.
Ig Isotype:
Mouse IgG1
Immunogen:
Lung cancer cell preparation
Cellular Localization:
Cytoplasm
Positive Control:
Skin, cervix.
Format:
1 mL culture supernatant solution containing
monoclonal antibody in 0.15 M PBS with protein
stabilizer and 0.02 sodium azide.
Storage:
Store at 4°C for short term. For long term
storage, aliquot and store at -20°C. Avoid
repeated freeze/thaw cycles.
Applications and Suggested Dilutions:

Immunohistochemistry:
Frozen sections: Fixation with acetone for 10
sec. at –20°C is recommended.
Paraffin-embedded tissue sections: Paraffin
material should be fixed with neutral formalin.
For such tissue sections, proteolytic
pretreatment with trypsin or pronase is
recommended. Working dilution: 1:5 –1:10.

Western blot
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.