Product: Anti-CREB pAb
Cat. No.: PC-359 (200 μL)
Background:
Transcription factor CREB binds the cAMP response
element (CRE) and activates transcription in response
t o a variety of extracellular signals including
neurotransmitters, hormones, membrane
depolarization, and growth or neurotrophic factors.
Protein kinase A and the calmodulin-dependent
protein kinase Ca MKII stimulate CREB
phosphorylation at Ser133, a key regulatory site
controlling transcriptional activity. Phosphorylation at
Ser 133 is also mediated by p44/42 MAP kinase,
p90RSK, p38 MAP kinase and MSK1. CREB
appears to play an important role in learning and
memory in both flies and mice.
Host:
Rabbit
Specificity:
Recognizes the 42 kDa CREB.
Species Reactivity:
Human, mouse and rat.
Format:
200 μL in immunaffinity-purified rabbit polyclonal
antibody in phosphate buffered saline (PBS)
containing 50% glycerol, 0.02% sodium azide
and protein stabilizer.
Storage:
Store at -20°C. For long term storage, aliquot
and store at -70°C. Avoid repeated freeze/thaw
cycles.
Applications and Suggested Dilutions:

Western blot: Use at a 1:200 - 1:1000 dilution.

Immunohistochemistry: Use at a 1:20 - 1:100
dilution.

Immunoprecipitation: Use at a 1:50 - 1:100
dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.