Product: Anti-Fas mAb, clone 2R2
Cat. No.: MC-121 (100 μg)
Synonyms:
CD95, APO-1, TNFRSF 6
Specificity:
This antibody is specific for human Fas.
Species Reactivity:
Human. Other species not tested.
Ig Isotype:
Mouse IgG3
Immunogen:
Mouse L cells stably over-expressing human
Fas.
Format:
100 μg monoclonal antibody at 1 mg/mL purified
from concentrated hybridoma tissue culture
supernatant. Prepared in PBS containing
protein stabilizer. Protein A-affinity purified.
Purity ³95% by SDS-PAGE.
Storage:
Store at 4°C, short term. Store at -20°C, long
term. Aliquot to avoid freeze/thaw.
Applications:

Functional activity: Induces apoptosis, no
cross-linking required. Can be used in DISC
(death-inducing signaling complex assays):
Co-immunoprecipitates FADD and caspase-8
after receptor activation.

Flow cytometry
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1. Induction of apoptosis in anti-Fas 2R2 stimulated
Jurkat T-cells.
Method: (A) Dose response: Jurkat cells (1x105) were
treated with agonistic anti-Fas 2R2 antibody at the indicated
concentrations for 24 h. Induction of apoptosis was
assessed by PI staining of hypodiploid nuclei and
subsequent flow cytometry.
(B) Time course of apoptosis: Jurkat cells (1x105) were
treated with 1 μg/mL anti-Fas 2R2. After the indicated time
points apoptosis was determined by FACS measurement of
hypodiploid DNA.
Figure 2. Detection of Fas surface expression by FACS
analysis.
Method: Normal L cells (open histogram) and cells stably
over-expressing human Fas (filled histogram) were
incubated with anti-Fas 2R2 and analyzed by indirect
immunofluorescence using PE-labeled secondary antibodies.
No immunostaining was obtained with an isotype matched
IgG control.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.