Product: Anti-chk1 kinase, Polyclonal
Cat. No: PC-175 (250 μg)
Description:
One mechanism by which checkpoints maintain
the fidelity of cell cycle events is through
blocking mitosis in response to unreplicated or
damaged DNA. In most species this occurs
through inhibiting activation of the cyclicdependent
kinase cdc2, which regulates entry
into mitosis. Chk1, a kinase involved in the DNA
damage checkpoint response can phosphorylate
Cdc25C an activator of cdc2. It is hypothesized
that chk1 induces serine 216 phosphorylation of
Cdc 25C and subsequent binding negatively
regulated Cdc25C, thus preventing it from
activating cdc2.
Specificity:
Recognizes chk1 kinase.
MW of antigen: 54 kDa.
Species Reactivity:
Human, monkey, mouse, and rat, other species
not tested.
Host: Sheep
Immunogen:
GST fusion protein corresponding to amino acid
sequence 1-474 of human chk1 kinase.
Cellular Localization: Cytoplasmic.
Format:
Provided as 0.2 μm sterile filtered solution in
PBS with 0.08% sodium azide.
Storage and Stability:
Store at -20°C. Aliquot to avoid freeze/thaw
cycles.
Applications and Suggested Dilutions:

Western blot: Use 1-10 μg/ml.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.